Composite

Part:BBa_K4343134

Designed by: Zheng Zhe   Group: iGEM22_NNU-China   (2022-10-09)


sgRNA4

he sgRNA expression frame consisting of the promoter of the transcribed RNA, the sgRNA scaffold and the corresponding N20 (sgRNA4-N20), which directs CBE-related elements to target TRP1 and complete base editing (C→T).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 365
    Illegal BsaI.rc site found at 629


[edit]
Categories
Parameters
None